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AGRICULTURE, AGROCHEMICAL, FEED PRODUCTION, AGROECOLOGY

METHOD OF STRENGTHENING SAND BY MICROBIOLOGICAL METHOD

Bauyrzhan Zhanatayev 1 , Zina Tukhyshbaeva 1 , Ainur Zhanataeva 2 , Meirzhan Dauletkul 3 , Ayman Toktamysova 4

1 Abai Kazakh National Pedagogical University; 2 KSE KEGEN District Hospital on PCV ; 3 Kazakh National Pedagogical University named after Abai; 4 Kazakh-Russian Medical University

doi.org/10.37884/4-2024/18 pp. 182-191 Admitted 22.10.2024 Published 30.12.2024

Abstract

         Cement is not only a building material, but also a material that ensures the preservation of cultural heritage in society. Although thousands of structures using cement concrete are built every year in the world, including cultural heritage and historical monuments, over time concrete is destroyed, becomes unusable and dangerous for humans and animals. Therefore, materials with the ability to self-repair can be used to preserve and restore these structures. The use of urease-active bacteria effectively solves these problems, because if bacteria are used in concrete structures, they continue to live and divide in concrete. Urease promotes the mineralization of calcium carbonate by hydrolysis of urea present in the environment. As a result of urease activity, carbon dioxide is released, bacteria can use urea as a nitrogen source, ammonia increases the pH of the medium, which leads to the formation of a precipitate of Ca2+ and Co3+ Ca2Co3. This unique property makes it particularly suitable for use in the construction industry (concrete structures, plaster mortars, ready-made mixtures, production of refractory elements, bricks).

        Microorganisms were isolated from wastewater and then tested for the ability to hydrolyze urea. According to the test results, 7 isolates were tested, 2 strains with high urease activity were selected from them.

       In this article, the method of bio-cementation of sand is studied. Rainwater erosion tests were carried out to test the strength of the hardened sand. In addition, when examining reinforced sand under the SEM-BCS microscope, it is possible to clearly see the pregnancy of calcium carbonates in the sand.

Biocement, urease resistance, sand, SEM microscope, MICP.

01 Introduction

The full text of the article is available for download in PDF format on the right panel.

02 References

  1. 1. Курманбаев, АА, Нагметова, ГЖ, Бижанова, ЛЖ, и соавт. 2017. Выделение уреолитическихъ бактерий, перспективных для микробиологического осаждения кальцита. Актуальные проблемы гуманитарных и естественных наук. 4(1). https://cyberleninka.ru/article/n/vydelenie-ureoliticheskih-bakteriy-perspektivnyh-dlya-mikrobiologicheskogo-osazhdeniya-kaltsita.
  2. 2. Сембаев, КД, Сембаева ДЖ, Данлыбаева ГА и соавт. 2019. Скрининг уреолитических микроорганизмов, перспективных для цементации песков. Проблемы Науки. 8 (141). https://cyberleninka.ru/article/n/skrining-ureoliticheskih-mikroorganizmov-perspektivnyh-dlya-tsementatsii-peskov.
  3. 3. Ahenkorah, I, Mizanur R., Rajibul K, et al. 2021. Enzyme induced calcium carbonate precipitation and its engineering application: A systematic review and meta-analysis. Construction and Building Materials. 308, 125000. https://www.sciencedirect.com/science/article/abs/pii/S0950061821027471
  4. 4. Akanksha, B, Emmanuel, S, Sumi, S. 2024. Composite biomediated engineering approaches for improving problematic soils: Potentials and opportunities, Science of The Total Environment. 914, 169808. https://www.sciencedirect.com/science/article/abs/pii/S0048969723084401
  5. 5. Alotaibi, E, Arab, MG, Abdallah, M, et al. 2022. Life cycle assessment of biocemented sands using enzyme induced carbonate precipitation (EICP) for soil stabilization applications. 12(1), 6032. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9001663/
  6. 6. Даулеткұл, М., Тұнғышбаева, З., Янкьевич, У., Қыдырбава, Ә., & Турсынханқызы, М. 2024. Деградациялану мен шөлейіттенуден қорғау үшін ферменттік индукцияланған кальций карбонатының тұнбасы арқылы құм мен тозған топырақты нығайту. Izdenister natigeler, (2 (102)), 271-282. https://journal.kaznaru.edu.kz/index.php/research/article/view/601
  7. 7. Joshi S., Goyal S., Mukherjee A., Reddy M.S. Microbial healing of cracks in concrete: a review. J. Ind. Microbiol. Biotechnol., 44(11), 1511‒1525, 2017. doi: 10.1007/s10295-017-1978-0 https://ouci.dntb.gov.ua/en/works/4knRppG7/
  8. 8. Castro M.J., Lopez C.E., Narayanasamy R., et al. Potential of enzymes (urease & carbonic anhydrase). Chim. Oggi. Chem., 34(4), 56‒59, 2016. https://www.researchgate.net/publication/306278739
  9. 9. Wang Z., Zhang N., Cai G., et al. Review of ground improvement using microbial induced carbonate precipitation (MICP). Mar.Georesour.Geotechnol, 35(8), 1135‒1146, 2017. doi: https://www.sciencedirect.com/science/article/pii/S2949929123000025
  10. 10. Mazzei L., Cianci M., Benini S., et al. Kinetic and structural studies reveal a unique binding mode of sulfite to the nickel center in urease. J. Inorg. Biochem., 154, 42‒49, 2016. doi: 10.1016/j.jinorgbio.2015.11.003 https://pubmed.ncbi.nlm.nih.gov/26580226/
  11. 11. Vsevolod, M, Elaman, K, Aibuldinov, K, et al. 2019. Efficient road base material from Kazakhstan's natural loam strengthened by ground cooled ferrous slag activated by lime production waste. Journal of Cleaner Production. 231, 1428-1436. https://www.sciencedirect.com/science/article/abs/pii/S095965261931786X
  12. 12. De Muynck W., Cox K., De Belie N., Verstraete W. Bacterial carbonate precipitation as an alternative surface treatment for concrete. Constr. Build. Mater., 22(5), 875‒885, 2018. doi: 10.1016/j.conbuildmat.2006.12.011 https://www.sciencedirect.com/science/article/pii/S2949929123000086
  13. 13. Basheer P.A.M., Basheer L., Cleland D.J., Long A.E. Surface treatments for concrete: assessment methods and reported performance. Constr. Build. Mater. 11(7‒8), 413‒429, 1997. https://www.sciencedirect.com/science/article/abs/pii/S0950061897000196
  14. 14. Zhang, K, Tang, CS, Jiang, NJ, et al. 2023. Microbial induced carbonate precipitation (MICP) technology: a review on the fundamentals and engineering applications. Environ Earth Sci. 82(9), 229. doi: 10.1007/s12665-023-10899-y. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10131530/
  15. 15. Krishnapriya S., Babu D.V. Isolation and identification of bacteria to improve the strength of concrete. Microbiol. Res., 174, 48‒55, 2015. doi: 10.1016/j.micres.2015.03.009 https://link.springer.com/article/10.1617/s11527-005-9014-7.
  16. 16. Garabito M.J., Márquez M.C., Ventosa A. Halotolerant Bacillus diversity in hypersaline environments. Can. J. Microbiol. 44(2), 95‒102, 1998. doi: 10.1139/w97-125 https://pubmed.ncbi.nlm.nih.gov/9783177/
  17. 17. Ventosa A., Márquez M.C., Garabito M.J., Arahal D.R. Moderately halophilic gram-positive bacterial diversity in hypersaline environments. Extremophiles, 2(3), 297‒304, 1998. https://pubmed.ncbi.nlm.nih.gov/8177169/
  18. 18. Panosyan H., Hakobyan A., Birkeland N.K., Trchounian A. Bacilli community of saline–alkaline soils from the Ararat Plain (Armenia) assessed by molecular and culture-based methods. Syst. Appl. Microbiol., 41(3), 232‒240, 2019. https://pubmed.ncbi.nlm.nih.gov/29342414/
  19. 19. Kushwaha B., Jadhav I., Verma H.N., et al. Betaine accumulation suppresses the de-novo synthesis of ectoine at a low osmotic concentration in Halomonas sp SBS 10, a bacterium with broad salinity tolerance. Mol. Biol. Rep., 46(5), 4779‒4786, 2019. https://pubmed.ncbi.nlm.nih.gov/31230183/
  20. 20. Obeidat M. Isolation and characterization of extremely halotolerant Bacillus species from Dead Sea black mud and determination of their antimicrobial and hydrolytic activities. Afr. J. Microbiol. Res., 11(32), 1303‒1314, 2017. doi: 10.5897/AJMR2017.8608 https://academicjournals.org/journal/AJMR/article-full-text-pdf/9A1272765714.pdf

Citation Links

[1]2024. METHOD OF STRENGTHENING SAND BY MICROBIOLOGICAL METHOD. Izdenister natigeler. 4 (104) (Dec. 2024), 182–191. DOI:https://doi.org/10.37884/4-2024/18.
(1)METHOD OF STRENGTHENING SAND BY MICROBIOLOGICAL METHOD. Izdenister natigeler 2024, No. 4 (104), 182-191. https://doi.org/10.37884/4-2024/18.
METHOD OF STRENGTHENING SAND BY MICROBIOLOGICAL METHOD. (2024). Izdenister Natigeler, 4 (104), 182-191. https://doi.org/10.37884/4-2024/18
METHOD OF STRENGTHENING SAND BY MICROBIOLOGICAL METHOD. Izdenister natigeler, [S. l.], n. 4 (104), p. 182–191, 2024. DOI: 10.37884/4-2024/18. Disponível em: https://natsus.kaznaru.edu.kz/index.php/research/article/view/745. Acesso em: 15 sep. 2026.
“METHOD OF STRENGTHENING SAND BY MICROBIOLOGICAL METHOD”. 2024. Izdenister Natigeler, no. 4 (104) (December): 182-91. https://doi.org/10.37884/4-2024/18.
“METHOD OF STRENGTHENING SAND BY MICROBIOLOGICAL METHOD” (2024) Izdenister natigeler, (4 (104), pp. 182–191. doi:10.37884/4-2024/18.
[1]“METHOD OF STRENGTHENING SAND BY MICROBIOLOGICAL METHOD”, Izdenister natigeler, no. 4 (104), pp. 182–191, Dec. 2024, doi: 10.37884/4-2024/18.
“METHOD OF STRENGTHENING SAND BY MICROBIOLOGICAL METHOD”. Izdenister Natigeler, no. 4 (104), Dec. 2024, pp. 182-91, https://doi.org/10.37884/4-2024/18.
“METHOD OF STRENGTHENING SAND BY MICROBIOLOGICAL METHOD”. Izdenister natigeler, no. 4 (104) (December 30, 2024): 182–191. Accessed September 15, 2026. https://natsus.kaznaru.edu.kz/index.php/research/article/view/745.
1.METHOD OF STRENGTHENING SAND BY MICROBIOLOGICAL METHOD. Izdenister natigeler [Internet]. 2024 Dec. 30 [cited 2026 Sep. 15];(4 (104):182-91. Available from: https://natsus.kaznaru.edu.kz/index.php/research/article/view/745
1.METHOD OF STRENGTHENING SAND BY MICROBIOLOGICAL METHOD. Izdenister natigeler. 2024;(4 (104):182-191. doi:10.37884/4-2024/18