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AGRICULTURE, AGROCHEMICAL, FEED PRODUCTION, AGROECOLOGY

INFLUENCE OF GROWTH REGULATORS ON THE MICROCLONAL PROPAGATION OF BLACKBERRY VARIETY HEAVEN CAN WAIT

Botakoz Tezekbayeva 1 , Altyn Khassein 1 , Khorlan Berkimbay 1 , Gulvira Yessenbayeva 2 , Maria Georgieva 3 , Natalya Malakhova 1

1 M.A. Aitkhozhin’s Institute of Molecular Biology and Biochemistry; 2 NJSC «Kazakh National Agrarian Research University» ; 3 Institute of Mountain Stockbreeding and Agriculture

doi.org/10.37884/2-2025/36 pp. 365-374 Admitted 17.04.2025 Published 30.06.2025

Abstract

This article presents the results of experiments aimed at studying the effect of various growth regulators (auxins, cytokinins, and gibberellins) on the microclonal propagation of the 'Heaven Can Wait' blackberry variety in vitro. The experiment examined different concentrations and combinations of growth hormones: BAP (6-benzylaminopurine), IBA (indole-3-butyric acid), NAA (naphthalene acetic acid), and GA3 (gibberellic acid). It was found that the highest multiplication rate (8.4) was achieved by the combined application of 1.0 mg/l BAP and 0.2 mg/l IBA. Data analysis revealed that IBA has a more pronounced stimulating effect on the formation of microshoots compared to NAA. Additionally, the introduction of GA3 into the nutrient medium generally reduced the multiplication rate, and this reduction was statistically significant. However, the combination of 1.0 mg/l BAP and 0.2 mg/l GA3 contributed to significant shoot elongation (up to 4.23 cm), which can be applied during the rooting and acclimatization stages. The results highlight the importance of selecting optimal concentrations of growth regulators for the microclonal propagation of specific blackberry varieties and may be used to further improve propagation protocols for 'Heaven Can Wait' with the aim of its widespread commercial introduction.

blackberry cultivar nutrient medium explants microclonal propagation growth regulators

01 Introduction

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02 References

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Citation Links

[1]2025. INFLUENCE OF GROWTH REGULATORS ON THE MICROCLONAL PROPAGATION OF BLACKBERRY VARIETY HEAVEN CAN WAIT. Izdenister natigeler. 2 (106) (Jun. 2025), 365–374. DOI:https://doi.org/10.37884/2-2025/36.
(1)INFLUENCE OF GROWTH REGULATORS ON THE MICROCLONAL PROPAGATION OF BLACKBERRY VARIETY HEAVEN CAN WAIT. Izdenister natigeler 2025, No. 2 (106), 365-374. https://doi.org/10.37884/2-2025/36.
INFLUENCE OF GROWTH REGULATORS ON THE MICROCLONAL PROPAGATION OF BLACKBERRY VARIETY HEAVEN CAN WAIT. (2025). Izdenister Natigeler, 2 (106), 365-374. https://doi.org/10.37884/2-2025/36
INFLUENCE OF GROWTH REGULATORS ON THE MICROCLONAL PROPAGATION OF BLACKBERRY VARIETY HEAVEN CAN WAIT. Izdenister natigeler, [S. l.], n. 2 (106), p. 365–374, 2025. DOI: 10.37884/2-2025/36. Disponível em: https://natsus.kaznaru.edu.kz/index.php/research/article/view/950. Acesso em: 15 sep. 2026.
“INFLUENCE OF GROWTH REGULATORS ON THE MICROCLONAL PROPAGATION OF BLACKBERRY VARIETY HEAVEN CAN WAIT”. 2025. Izdenister Natigeler, no. 2 (106) (June): 365-74. https://doi.org/10.37884/2-2025/36.
“INFLUENCE OF GROWTH REGULATORS ON THE MICROCLONAL PROPAGATION OF BLACKBERRY VARIETY HEAVEN CAN WAIT” (2025) Izdenister natigeler, (2 (106), pp. 365–374. doi:10.37884/2-2025/36.
[1]“INFLUENCE OF GROWTH REGULATORS ON THE MICROCLONAL PROPAGATION OF BLACKBERRY VARIETY HEAVEN CAN WAIT”, Izdenister natigeler, no. 2 (106), pp. 365–374, Jun. 2025, doi: 10.37884/2-2025/36.
“INFLUENCE OF GROWTH REGULATORS ON THE MICROCLONAL PROPAGATION OF BLACKBERRY VARIETY HEAVEN CAN WAIT”. Izdenister Natigeler, no. 2 (106), June 2025, pp. 365-74, https://doi.org/10.37884/2-2025/36.
“INFLUENCE OF GROWTH REGULATORS ON THE MICROCLONAL PROPAGATION OF BLACKBERRY VARIETY HEAVEN CAN WAIT”. Izdenister natigeler, no. 2 (106) (June 30, 2025): 365–374. Accessed September 15, 2026. https://natsus.kaznaru.edu.kz/index.php/research/article/view/950.
1.INFLUENCE OF GROWTH REGULATORS ON THE MICROCLONAL PROPAGATION OF BLACKBERRY VARIETY HEAVEN CAN WAIT. Izdenister natigeler [Internet]. 2025 Jun. 30 [cited 2026 Sep. 15];(2 (106):365-74. Available from: https://natsus.kaznaru.edu.kz/index.php/research/article/view/950
1.INFLUENCE OF GROWTH REGULATORS ON THE MICROCLONAL PROPAGATION OF BLACKBERRY VARIETY HEAVEN CAN WAIT. Izdenister natigeler. 2025;(2 (106):365-374. doi:10.37884/2-2025/36