Login | Register
Kazakh National Agrarian Research University ISSN 2304-3334 (Print)  ·  ISSN 2304-3334 (Online)
submit an article
Menu
submit an article
AGRICULTURE, AGROCHEMICAL, FEED PRODUCTION, AGROECOLOGY

MICROCLONAL PR OPAGATION OF COMMON ALMOND (PRUNUS DULCIS MILL) FOR THE RESTORATION OF REGRESSIVE POPULATIONS

Timur Turdiyev 1 , Saule Baizhumanova 1 , Kamila Yemesheva 1 , Natalya Mikhailenko 2 , Irina Kovalchuk 2

1 NJSC «Kazakh National Agrarian Research University» ; 2 Institute of Plant Biology and Biotechnology

doi.org/10.37884/3-2024/20 pp. 178-187 Admitted 26.07.2024 Published 30.09.2024

Abstract

The Common Almond (Prunus Dulcis Mill) represents an economic value due to the nutritional value of its fruit: Taste, nutrient-rich and long-term storage. In the territory of the Republic of Kazakhstan, the almond is classified as an endangered population, which makes it necessary to restore them.

The recovery of the declining populations of the Common Almond is efficiently carried out with the application of in vitro culture, which allows to obtain fast and mass-quantity planting material. For the production of aseptic plants, it is better to use green shoots with 2-3 buds, which are grown from lignified shoots. Effective method of sterilization from saprophytic microflora - consecutive treatment with water solution of bleach «White» (1:1) for 10 minutes, solution 0,1% HgCl2 5 min, 3 times sterile distilled water. Optimal medium for in vitro - hormone-free MC, for clonal micropropagation - MC containing 1.0 mg/l BAP, 0.02 mg/l IBA and 30 g/l sucrose. The reproduction rate is 2.8 on average, and the length of shoots in 4-6 weeks - 5.0-6.3 cm.

common almond, aseptic plants, clonal micropropagation, in vitro, shoots, sterilization, regeneration of shoots.

01 Introduction

The full text of the article is available for download in PDF format on the right panel.

02 References

  1. 1 Каирова М.Ж. Интродуцированные виды орехов и их популяции в Казахстане [Текст]/ М.Ж. Каирова // Научные труды Чебоксарского филиала Главного ботанического сада им. Н.В. Цицина РАН. – 2019. – № 13. – С. 115-121.
  2. 2 Материалы Шестого национального доклада Республики Казахстан о биологическом разнообразии [Текст]. – Астана. – 2018.
  3. 3 Зверев Н.Е. Оценка плодов фисташки, выделенных в популяциях, произрастающих в Казахстане [Текст] / Н.Е. Зверев, К.Р. Калыбаев, А.А. Курмантаева // Известия Национальной академии наук Республики Казахстан. Серия биологическая и медицинская. – 2017. – № 5. – С. 5-23.
  4. 4 Ben Khadher T. Phytochemical Profiling and Biological Potential of Prunus dulcis Shell Extracts. [Text] / T. Ben Khadher, S. Sassi-Aydi, S. Aydi, M. Mars, J. Bouajila // Plants. – 2023. – No. 12 (14). – Р. 27-33. https://doi.org/10.3390/plants12142733.
  5. 5 Болотова А.С. Химический состав орехов интродуцированных сортов сладкого миндаля в южном Кыргызстане [Текст] / А.С.Болотова // Sciences of Europe. – 2017. – № 13-1 (13). – С. 8-12.
  6. 6 Сулейманова С.Д. Микроклональное размножение плодовых культур [Текст] / С.Д. Сулейманова. // Восточно-европейский научный журнал. – 2016. – 11 (2). – С. 47-54.;
  7. 7 Арзуманов В.А. Растительные ресурсы плодовых и орехоплодных растений Центральной Азии и их роль в формировании местного сортимента [Текст] / В.А. Арзуманов, Е.А. Бутков, М.К. Турдиева, К.И. Байметов, А.А. Юшев // Bioversity International. – Италия. – 2015. – С. 106.
  8. 8 Ettore B., Stefano P., Van Mele P. Descriptors for Pistachio (Pistacia vera L.) [Text] / B. Ettore, P. Stefano, P. Van Mele – Рим, Италия. – 1997. – 63 р. / Перевод Турдиевой М., Гулямовой Х. с английского издания. – 2002. – 63 с.;
  9. 9 International Board for Plant Genetic Resources (IBPGR) / Descriptors list for almond (Prunus amygdalus) (Revised). International Board for Plant Genetic Resources (IBPGR). – 1985. – 30 p.
  10. 10 Ташкенбаева А.К. Производство оздоровленного посадочного материала земляники садовой лучших сортов мировой коллекции для создания оригинальных базисных маточников [Текст] / А.К. Ташкенбаева, М.Ж. Саршаева, Ж.М. Матай // Ізденістер, нәтижилер – Исследования, результаты. – 2023. – № 4 (100). – С. 159–166. https://doi.org/10.37884/4-2023/18
  11. 11 Сембеков М.Т. Влияние фитостимуляторов во взаимодействии со световым освещением на рост и развитие микроклонов селитрянки [Текст] / М.Т. Сембеков, Е.А. Шаденова, М.А. Кайгермазова, Л.Б. Ашикова, Э.Д. Джангалина, Терлецкая Н.В. // Ізденістер, нәтижелер - Исследования, результаты. – 2024. – № 1 (101). – С. 202–209. https://doi.org/10.37884/1-2024/20
  12. 12 Viss P.R. A simplified method for the control of bacterial contamination in woody plant tissue culture / Brooks E.M., Driver J.A. // In Vitro Cell. Dev. Biol. – 1991. – V. 27. – P. 42.
  13. 13 Серафимович М.В. Влияние состава питательной среды на приживаемость эксплантов миндаля Ледебуровского (Amygdalus ledebouriana Schlecht) в культуре in vitro [Текст] / Серафимович М.В., Кириллов В.Ю., Стихарева Т.Н. // Вестник Государственного университета имени Шакарима. – 2020. – № 3 (91). – С. 289-293.
  14. 14 Isikalan Ç. In vitro micropropagation of almond (Amygdalus communis L. cv. Nonpareil) / Isikalan Ç., Akbas F.A., Namli S., Tilkat E., Basaran D. // African Journal of Biotechnology. – 2008. – Vol. 7 (12). – P. 1875-1880. DOI: 10.5897/AJB2008.000-5035

Citation Links

[1]2024. MICROCLONAL PR OPAGATION OF COMMON ALMOND (PRUNUS DULCIS MILL) FOR THE RESTORATION OF REGRESSIVE POPULATIONS. Izdenister natigeler. 3(103) (Sep. 2024), 178–187. DOI:https://doi.org/10.37884/3-2024/20.
(1)MICROCLONAL PR OPAGATION OF COMMON ALMOND (PRUNUS DULCIS MILL) FOR THE RESTORATION OF REGRESSIVE POPULATIONS. Izdenister natigeler 2024, No. 3(103), 178-187. https://doi.org/10.37884/3-2024/20.
MICROCLONAL PR OPAGATION OF COMMON ALMOND (PRUNUS DULCIS MILL) FOR THE RESTORATION OF REGRESSIVE POPULATIONS. (2024). Izdenister Natigeler, 3(103), 178-187. https://doi.org/10.37884/3-2024/20
MICROCLONAL PR OPAGATION OF COMMON ALMOND (PRUNUS DULCIS MILL) FOR THE RESTORATION OF REGRESSIVE POPULATIONS. Izdenister natigeler, [S. l.], n. 3(103), p. 178–187, 2024. DOI: 10.37884/3-2024/20. Disponível em: https://natsus.kaznaru.edu.kz/index.php/research/article/view/674. Acesso em: 15 sep. 2026.
“MICROCLONAL PR OPAGATION OF COMMON ALMOND (PRUNUS DULCIS MILL) FOR THE RESTORATION OF REGRESSIVE POPULATIONS”. 2024. Izdenister Natigeler, no. 3(103) (September): 178-87. https://doi.org/10.37884/3-2024/20.
“MICROCLONAL PR OPAGATION OF COMMON ALMOND (PRUNUS DULCIS MILL) FOR THE RESTORATION OF REGRESSIVE POPULATIONS” (2024) Izdenister natigeler, (3(103), pp. 178–187. doi:10.37884/3-2024/20.
[1]“MICROCLONAL PR OPAGATION OF COMMON ALMOND (PRUNUS DULCIS MILL) FOR THE RESTORATION OF REGRESSIVE POPULATIONS”, Izdenister natigeler, no. 3(103), pp. 178–187, Sep. 2024, doi: 10.37884/3-2024/20.
“MICROCLONAL PR OPAGATION OF COMMON ALMOND (PRUNUS DULCIS MILL) FOR THE RESTORATION OF REGRESSIVE POPULATIONS”. Izdenister Natigeler, no. 3(103), Sept. 2024, pp. 178-87, https://doi.org/10.37884/3-2024/20.
“MICROCLONAL PR OPAGATION OF COMMON ALMOND (PRUNUS DULCIS MILL) FOR THE RESTORATION OF REGRESSIVE POPULATIONS”. Izdenister natigeler, no. 3(103) (September 30, 2024): 178–187. Accessed September 15, 2026. https://natsus.kaznaru.edu.kz/index.php/research/article/view/674.
1.MICROCLONAL PR OPAGATION OF COMMON ALMOND (PRUNUS DULCIS MILL) FOR THE RESTORATION OF REGRESSIVE POPULATIONS. Izdenister natigeler [Internet]. 2024 Sep. 30 [cited 2026 Sep. 15];(3(103):178-87. Available from: https://natsus.kaznaru.edu.kz/index.php/research/article/view/674
1.MICROCLONAL PR OPAGATION OF COMMON ALMOND (PRUNUS DULCIS MILL) FOR THE RESTORATION OF REGRESSIVE POPULATIONS. Izdenister natigeler. 2024;(3(103):178-187. doi:10.37884/3-2024/20