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AGRICULTURE, AGROCHEMICAL, FEED PRODUCTION, AGROECOLOGY

SELECTION OF CONDITIONS FOR OBTAINING ECOLOGICALLY PURE DRY PROBIOTIC AGAINST MIXED INTESTINAL INFECTION FOR FARM ANIMALS

Жанар Сагидолдина 1 , Эльнара Куандыкова 1

1 Kazakh National Agrarian Research University

doi.org/10.37884/2-2023/15 pp. 154-160 Admitted 30.03.2023 Published 29.06.2023

Abstract

As a result of the study, the best viability of monocultures and associations under freeze drying has been determined. The best two protective media during storage and their composition are the following: media No.1 includes (%) sodium acetate - 2.5; sodium citrate - 2.5; sucrose - 10.0; and protective media No.2 containштп (%) sucrose 8.0; gelatin - 1.5; skim milk - 5.0. Thus the titer of bacteria equaled to values of 6.0x1010 and 4.2x1010 CFU / g, respectively.

When determining the stability of dried preparations during storage of probiotic bacteria and their association, the best protective medium is No. 2 (sucrose 8.0%; gelatin - 1.5%; skim milk - 5.0%).

The antagonistic activity of monocultures (lactic acid bacteria Lactobacillus plantarum1, Streptococcus salivarius 20n, Lactobacillus fermentum 15, propionic acid bacteria Propionibacterium shermanii 34) and associations based on these monocultures is preserved practically at the initial level upon freeze drying.

A probiotic based on lactic acid and propionic acid bacteria can be used as a therapeutic and prophylactic agent against dyspepsia for farm animals. At the same time, there is no need for the use of antibiotics and other antibacterial agents for the treatment of diseased animals.

probiotics, bifidobacteria, lactobacilli, antagonism, immunostimulating effect, antibiotics, polyresistance, dysbiosis, intestinal infections, extra-intestinal infections

01 Introduction

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02 References

  1. 1. Danilevskaya N.V., Sidorov M.A., Subbotin V.V. Probiotics in veterinary medicine // Veterinary medicine. - 2002. - No. 11.
  2. 2. Probiotics. Achievements and prospects of use in animal husbandry / B.V. Tarakanov, T.A. Nikolicheva, V.V. Aleshin et al. // Past, present and future of zootechnical science: Tr. VIZHA. - 2004. - Vol. 3. - Issue. 62 .-- P. 69−73.
  3. 3. Panin A.N., Malik N.I. Probiotics - an integral component of rational animal feeding // Veterinary medicine. - 2006. - No. 7. - P. 19–22.
  4. 4. Anadun A., Martínez-Larranaga M. R., Aranzazu-Martínez M. Probiotics for animal nutrition in the European Union. Regulation and Safety Assessment. Regulatory Toxicology // Pharmacology. - 2006. - Vol. 12. - P. 91–95.
  5. 5. Ovsyannikov Yu.S. Probiotics in veterinary medicine / Yu.S. Ovsyannikov, G.I. Tikhonov, O.V. Golunova // Veterinary Medicine. - 2009. - No. 1-2. - P. 66-68.
  6. 6. Ja Kyeom Seo, Seon-Woo Kim1, Myung Hoo Kim, Santi D. Upadhaya, Dong Keun Kam2 and Jong K. Ha Direct-fed Microbials for Ruminant Animals Asian-Aust // J. Anim. Sci. - 2010. - Vol. 23, no. 12. - P. 1657-1667.
  7. 7.Bondarenko V.M. Molecular-cellular mechanisms of the therapeutic action of probiotics / V.M. Bondarenko // BIOPreparations. - 2010. - № 1. - [Electronic resource] - Access mode. - URL:
  8. 8. https://argo-tema.ru/article-10719.html
  9. 9. Selection of a nutrient medium for growing bacteria which are part of an environmentally friendly veterinary probiotic preparation. N e w s of the national academy of sciences of the republic of kazakhstan series of biological and medical Volume 3, Number 339 (2020), C. 29 – 34 - http://biological-medical.kz/images/pdf/b20203/29-34.pdf
  10. 10. N.N. Gavrilova, 1A.K. Sadanov, I.A. Ratnikova, Zh.E. Sagidoldina. Testing of an environmentally friendly probiotic drug against dyspepsia of calves and lambs// Izvestiya NAS RK, ser. biol. and med. – 2020. - No. 4. http://agricultural.kz/assets/20204/5-11.pdf
  11. 11. Sagidoldina Zhanar, Sadanov Amankeldi, Ratnikova Irina, Kuandykova Elnara and 1Tokkozhaeva Meruert Selection of Probiotic Microorganisms Against Pathogens of Agricultural Animals DOI: https://doi.org/10.3844/ojbsci.2021.299.303

Citation Links

[1]2023. SELECTION OF CONDITIONS FOR OBTAINING ECOLOGICALLY PURE DRY PROBIOTIC AGAINST MIXED INTESTINAL INFECTION FOR FARM ANIMALS. Izdenister natigeler. 2 (98) (Jun. 2023), 154–160. DOI:https://doi.org/10.37884/2-2023/15.
(1)SELECTION OF CONDITIONS FOR OBTAINING ECOLOGICALLY PURE DRY PROBIOTIC AGAINST MIXED INTESTINAL INFECTION FOR FARM ANIMALS. Izdenister natigeler 2023, No. 2 (98), 154-160. https://doi.org/10.37884/2-2023/15.
SELECTION OF CONDITIONS FOR OBTAINING ECOLOGICALLY PURE DRY PROBIOTIC AGAINST MIXED INTESTINAL INFECTION FOR FARM ANIMALS. (2023). Izdenister Natigeler, 2 (98), 154-160. https://doi.org/10.37884/2-2023/15
SELECTION OF CONDITIONS FOR OBTAINING ECOLOGICALLY PURE DRY PROBIOTIC AGAINST MIXED INTESTINAL INFECTION FOR FARM ANIMALS. Izdenister natigeler, [S. l.], n. 2 (98), p. 154–160, 2023. DOI: 10.37884/2-2023/15. Disponível em: https://natsus.kaznaru.edu.kz/index.php/research/article/view/249. Acesso em: 15 sep. 2026.
“SELECTION OF CONDITIONS FOR OBTAINING ECOLOGICALLY PURE DRY PROBIOTIC AGAINST MIXED INTESTINAL INFECTION FOR FARM ANIMALS”. 2023. Izdenister Natigeler, no. 2 (98) (June): 154-60. https://doi.org/10.37884/2-2023/15.
“SELECTION OF CONDITIONS FOR OBTAINING ECOLOGICALLY PURE DRY PROBIOTIC AGAINST MIXED INTESTINAL INFECTION FOR FARM ANIMALS” (2023) Izdenister natigeler, (2 (98), pp. 154–160. doi:10.37884/2-2023/15.
[1]“SELECTION OF CONDITIONS FOR OBTAINING ECOLOGICALLY PURE DRY PROBIOTIC AGAINST MIXED INTESTINAL INFECTION FOR FARM ANIMALS”, Izdenister natigeler, no. 2 (98), pp. 154–160, Jun. 2023, doi: 10.37884/2-2023/15.
“SELECTION OF CONDITIONS FOR OBTAINING ECOLOGICALLY PURE DRY PROBIOTIC AGAINST MIXED INTESTINAL INFECTION FOR FARM ANIMALS”. Izdenister Natigeler, no. 2 (98), June 2023, pp. 154-60, https://doi.org/10.37884/2-2023/15.
“SELECTION OF CONDITIONS FOR OBTAINING ECOLOGICALLY PURE DRY PROBIOTIC AGAINST MIXED INTESTINAL INFECTION FOR FARM ANIMALS”. Izdenister natigeler, no. 2 (98) (June 29, 2023): 154–160. Accessed September 15, 2026. https://natsus.kaznaru.edu.kz/index.php/research/article/view/249.
1.SELECTION OF CONDITIONS FOR OBTAINING ECOLOGICALLY PURE DRY PROBIOTIC AGAINST MIXED INTESTINAL INFECTION FOR FARM ANIMALS. Izdenister natigeler [Internet]. 2023 Jun. 29 [cited 2026 Sep. 15];(2 (98):154-60. Available from: https://natsus.kaznaru.edu.kz/index.php/research/article/view/249
1.SELECTION OF CONDITIONS FOR OBTAINING ECOLOGICALLY PURE DRY PROBIOTIC AGAINST MIXED INTESTINAL INFECTION FOR FARM ANIMALS. Izdenister natigeler. 2023;(2 (98):154-160. doi:10.37884/2-2023/15