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STOCK-RAISING AND VETERINARY

ISOLATION OF BACTERIOPHAGES AGAINST THE CAUSATIVE AGENT OF HORSE STRANGLES -STREPTOCOCCUS EQUI AND STUDY OF THEIR BIOLOGICAL PROPERTIES

B.A* Yespembetov 1 , Е.А. Bulatov 1 , М.К. Sarmykova 1 , E.B. Serikbay 1 , А.А. Sambetbaev 2

1 RSE «Research Institute of biological safety problems»; 2 Kazakh National Agrarian Research University

doi.org/10.37884/2-2021/2 pp. 17-26 Admitted 03.03.2022 Published 30.06.2021

Abstract

The study of the biological properties of the washing phage is an important stage in the creation of biological products, phagoindication and identification of bacteria. The main sign of the phage's effect on sensitive bacteria is their lysis, accompanied by the release of new phage virions into the environment.
The novelty of this work is that for the first time in Kazakhstan, research is being conducted to study the biological properties of bacteriophages for the treatment of horse soap, isolated environmental objects and biomaterial from farms in the Almaty region.
All the studied phages had a titer of 107-109 according to Appelmann and 109-1010 according to
Grazia, had a pronounced specificity in relation to Streptococcus equi and did not show activity against Streptococcus aureus and Escherichia coli, they retained lytic activity for 2 months, were resistant to heating in the range of 60°C-95°C for 30 minutes. The phages were resistant to 10% chloroform solution for 45 min.

horse soap, bacteriophage, biological material, test strains, Streptococcus equi, environmental objects, mutation, vaccine.

01 Introduction

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02 References

  1. 1. Sansyzbaev A.R. Myt loshadei v Kazakhstane, (rasprostranenie, svoistva vozbuditelia, razrabotka sredstv spetsificheskoi profilaktiki i lecheniia). Avtoref. Dis. d-ra vet.nauk.- M., 1993. - S. 41.
  2. 2. Bizhanov A.B. Rol streptokokkov gruppy S v infektsionnoi patologii loshadei / A.B. Bizhanov, A.R. Sansyzbaev, Sh.O. Tokeev // Vestn. Selkhoznauki Kazakhstana. 1994. - №6. - S. 121-123.
  3. 3. Kaiypbai B.B. Lechenie smeshannogo techeniia myta i pasterelleza loshadei: avtoref. … kand. vet. nauk. –Almaty, 2003. –27 s.
  4. 4. Lindahl Susanne. Streptococcus equi subsp. equi and Streptococcus equi subsp. Zooepidemicus. Doctoral Thesis. Swedish University of Agricultural Sciences. U., 2013. С. 75.
  5. 5. Bakteriofagi: biologiia i primenenie /Red.: Katter E., A. Sulakvelidze. M.: Nauchnyi mir. 2012.
  6. 6. Espembetov B.A., Syrym N.S., Shestakov A.G., Vasilev D.A. Monografiia: «Prakticheskoe primenenie bakteriofagov na territorii RK». Ulianovsk. 2019 g. 624 s.
  7. 7. Materialy mezhdunarodnoi nauchno-prakticheskoi konferentsii «Bakteriofagi: Teoreticheskie i prakticheskie aspekty primeneniia v meditsine, veterinarii i pishchevoi promyshlennosti» / – Ulianovsk: GSKhA im. P.A. Stolypina, 2013. – Т. II – 182 s.
  8. 8. Syrym N.S., Espembetov B.A. Podbor pitatelnykh sred dlia vydeleniia mikobakteriofagov. «Іzdenіster, nətizheler – Issledovaniia, rezultaty». №1(77) 2018. ISSN 2304-334-02. S.483-488.
  9. 9. Syrym N.S., Espembetov B.A., Zinina N.N., Sansyzbai A.R., Nusupova S.T. Biologicheskie i molekuliarno-geneticheskie svoistva epizooticheskoi kultury streptococcus equi, vydelennoi iz patologicheskogo materiala zherebenka. «Іzdenіster, nətizheler – Issledovaniia, rezultaty». №4, 2020. ISSN 2304-334-02. S.82-88.

Citation Links

[1]2021. ISOLATION OF BACTERIOPHAGES AGAINST THE CAUSATIVE AGENT OF HORSE STRANGLES -STREPTOCOCCUS EQUI AND STUDY OF THEIR BIOLOGICAL PROPERTIES. Izdenister natigeler. 2 (90) (Jun. 2021), 17–26. DOI:https://doi.org/10.37884/2-2021/2.
(1)ISOLATION OF BACTERIOPHAGES AGAINST THE CAUSATIVE AGENT OF HORSE STRANGLES -STREPTOCOCCUS EQUI AND STUDY OF THEIR BIOLOGICAL PROPERTIES. Izdenister natigeler 2021, No. 2 (90), 17-26. https://doi.org/10.37884/2-2021/2.
ISOLATION OF BACTERIOPHAGES AGAINST THE CAUSATIVE AGENT OF HORSE STRANGLES -STREPTOCOCCUS EQUI AND STUDY OF THEIR BIOLOGICAL PROPERTIES. (2021). Izdenister Natigeler, 2 (90), 17-26. https://doi.org/10.37884/2-2021/2
ISOLATION OF BACTERIOPHAGES AGAINST THE CAUSATIVE AGENT OF HORSE STRANGLES -STREPTOCOCCUS EQUI AND STUDY OF THEIR BIOLOGICAL PROPERTIES. Izdenister natigeler, [S. l.], n. 2 (90), p. 17–26, 2021. DOI: 10.37884/2-2021/2. Disponível em: https://natsus.kaznaru.edu.kz/index.php/research/article/view/16. Acesso em: 15 sep. 2026.
“ISOLATION OF BACTERIOPHAGES AGAINST THE CAUSATIVE AGENT OF HORSE STRANGLES -STREPTOCOCCUS EQUI AND STUDY OF THEIR BIOLOGICAL PROPERTIES”. 2021. Izdenister Natigeler, no. 2 (90) (June): 17-26. https://doi.org/10.37884/2-2021/2.
“ISOLATION OF BACTERIOPHAGES AGAINST THE CAUSATIVE AGENT OF HORSE STRANGLES -STREPTOCOCCUS EQUI AND STUDY OF THEIR BIOLOGICAL PROPERTIES” (2021) Izdenister natigeler, (2 (90), pp. 17–26. doi:10.37884/2-2021/2.
[1]“ISOLATION OF BACTERIOPHAGES AGAINST THE CAUSATIVE AGENT OF HORSE STRANGLES -STREPTOCOCCUS EQUI AND STUDY OF THEIR BIOLOGICAL PROPERTIES”, Izdenister natigeler, no. 2 (90), pp. 17–26, Jun. 2021, doi: 10.37884/2-2021/2.
“ISOLATION OF BACTERIOPHAGES AGAINST THE CAUSATIVE AGENT OF HORSE STRANGLES -STREPTOCOCCUS EQUI AND STUDY OF THEIR BIOLOGICAL PROPERTIES”. Izdenister Natigeler, no. 2 (90), June 2021, pp. 17-26, https://doi.org/10.37884/2-2021/2.
“ISOLATION OF BACTERIOPHAGES AGAINST THE CAUSATIVE AGENT OF HORSE STRANGLES -STREPTOCOCCUS EQUI AND STUDY OF THEIR BIOLOGICAL PROPERTIES”. Izdenister natigeler, no. 2 (90) (June 30, 2021): 17–26. Accessed September 15, 2026. https://natsus.kaznaru.edu.kz/index.php/research/article/view/16.
1.ISOLATION OF BACTERIOPHAGES AGAINST THE CAUSATIVE AGENT OF HORSE STRANGLES -STREPTOCOCCUS EQUI AND STUDY OF THEIR BIOLOGICAL PROPERTIES. Izdenister natigeler [Internet]. 2021 Jun. 30 [cited 2026 Sep. 15];(2 (90):17-26. Available from: https://natsus.kaznaru.edu.kz/index.php/research/article/view/16
1.ISOLATION OF BACTERIOPHAGES AGAINST THE CAUSATIVE AGENT OF HORSE STRANGLES -STREPTOCOCCUS EQUI AND STUDY OF THEIR BIOLOGICAL PROPERTIES. Izdenister natigeler. 2021;(2 (90):17-26. doi:10.37884/2-2021/2