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AGRICULTURE, AGROCHEMICAL, FEED PRODUCTION, AGROECOLOGY

OPTIMIZATION OF THE CLONAL MICROPROPAGATION METHOD FOR OBTAINING VI RUS-FREE PLANTING MATERIAL OF GARDEN STRAWBERRIES (FRAGARIA×ANANASSA)

Akerke Tashkenbayeva 1 , Moldir Sarshayeva 2 , Ilya Korotetsky 3 , Saule Kazybaeva 1

1 Kazakh Scientific Research Institute of Fruit and Vegetable Growing, International Engineering and Technology University, Almaty, Kazakhstan; 2 Kazakh Scientific Research Institute of Fruit and Vegetable Growing, Almaty, Kazakhstan; 3 Scientific Center for Anti-Infectious Drugs, Almaty, Kazakhstan

doi.org/10.37884/1-2026/10 pp. 93-105 Admitted 01.10.2025 Published 27.02.2026

Abstract

The article presents the results of a study aimed at optimizing the biotechnology of micro clonal propagation of three varieties of Fragaria × ananassa in order to obtain healthy, virus-free planting ma terial. The novelty of the study lies in the development of an integrated biotechnological approach to obtaining virus-free strawberry planting material using a new synergistic combination of metabolically active addi tives-proline, adenosine triphosphate (ATP) and ascorbic acid. The proposed strategy reduces stress responses in explants during early cultivation stages, enhances energy metabolism and stabilizes meristem cells, in creasing micropropagation rate and regenerative capacity compared to traditional protocols. This combination was first applied to improve the morphogenetic activity of strawberry explants and demonstrated a sustained 30–45 % increase in reproduction rate depending on genotype. Methodologically, the study included stages of sterilization, regeneration, proliferation, and rooting of apical meristems. It was found that a five-minute treat ment with a 0.1 % sodium hypochlorite (NaClO) solution provides an optimal balance between sterilizations efficiency and tissue survival, reducing the contamination level to 25–35 % with meristem viability of 65–75 %. The addition of ascorbic acid (10 mg/L) prevented phenolic darkening of the meristems. The inclusion of proline and ATP contributed to an increase in the length of micro-rosettes and the reproduction coefficient: in Malwina variety – from 1:11 to 1:16, in Black Prince variety – from 1:7 to 1:11, and in the Sabrina variety – from 1:3 to 1:4. Molecular biological analysis using RT-PCR confirmed the absence of SVBV, SCV, SMoV, SPaV and BPYV viruses, indicating a high degree of recovery of the material obtained. In conclusion, it has been established that the proposed microclonal propagation protocol increases the regenerative capacity of meristems, accelerates the production of virus-free plants, and is recommended for industrial production of strawberry seedlings.

strawberry, regeneration, nutrient medium, microclonal propagation, viruses, tissue culture, in vitro

01 Introduction

The full text of the article is available for download in PDF format on the right panel.

02 References

  1. Stegmeir T.L. (2010). Performance of an elite strawberry population derived from wild germplasm of fragaria chiloensis and F. VIRGINIANA. – Michigan State University, Рр.1140. https://doi.org/10.21273/hortsci.45.8.1140
  2. Thompson J.R., Jelkmann W. (2003). The detection and variation of strawberry mottle virus //Plant disease. Т. 87. №. 4. Pp. 385–390. https://doi.org/10.1094/PDIS.2003.87.4.385
  3. Martin R.R., Tzanetakis I.E. (2006). Characterization and recent advances in detection of strawberry viruses //Plant disease. Т. 90. №. 4. Pp. 384– 396. https://doi.org/10.1094/PD-90-0384
  4. Xiang Y., Bernardy M., Bhagwat B., Wiersma P.A., DeYoung R., Bouthillier M. (2015). The complete genome sequence of a new polerovirus in strawberry plants from eastern canada showing strawberry decline symptoms //Archives of virology. Т. 160. №. 2. Pp. 553–556. https://doi.org/10.1007/s00705-014-2267-0
  5. A.K Tashkenbaeva, M.J Sarshaeva, J.M. Matai (2023). Túpnusqa negіzgі analyqbaq qurý úshіn baqsha qulpynaiynyń álemdіk kollektsiianyń eń jaqsy sorttarynan saýyqtyrylǵan otyrǵyzý materialyn óndіrý. //izdenister, natigeler. №4(100) Pp.159. DOI: https://doi.org/10.37884/4-2023/18 [in Kaz]
  6. Trofimova O, Batyrshin T. (2023). Internet resources [Text] https://informburo.kz/fotoreportazh/zemlyanika-limony-kapusta-na-cyom-zarabatyvayut-kazaxstanskie-fermery [in Russ]
  7. Murashige T., Skoog F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures //Physiologia plantarum. Т. 15. №. 3. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  8. Baimýhametova E.A., Kýlýev B.R. (2020). Potemnenie rastitelnyh tkanei pri kýltivirovanii in vitro i sposoby ego predotvraeniia// Biotehnologiia. T. 36. №2. Pp.26–42. DOI: https://doi.org/10.21 519/0234-2758-2020-36-2-26-42 [in Russ]
  9. EIKichaoui A.Y. (2014). In vitro propagation of strawberry (fragaria× annanasa duch.) through organogenesis via runner tips //Ann. Plant Sci. Т. 3. №. 3. Pp. 619– 627.
  10. Mukherjee E., Gantait S. (2024). Strawberry biotechnology: a review on progress over past 10 years //Scientia Horticulturae. Т. 338. Pp. 113618. https://doi.org/10.1016/j.scienta.2024.113618
  11. Sharma, N., Kumar, N., James, J., Kalia, S., & Joshi, S. (2023). Strategies for successful acclimatization and hardening of in vitro regenerated plants: challenges and innovations in micropropagation techniques // Plant Science Today (Early Access). Pp.1–6. https://doi.org/10.14719/pst.2376
  12. Krakhmaleva, I.L., Molkanova, O.I., Orlova, N.D., Koroleva, O.V., Mitrofanova, I.V. (2024). In vitro morpho-anatomical and regeneration features of cultivars of actinidia kolomikta (maxim.) maxim // Horticulturae. Т. 10. №. 12. Pp. 1335. https://doi.org/10.3390/horticulturae10121335
  13. Tashkenbayeva A.K., Sarshayeva M.Z. (2023). Propagation of certified fragaria ananassa in tissue culture // I International Symposium on Plant Propagation, Nursery Organization and Management for the Production of Certified Fruit Trees 1413. Pp. 59– 66. https://doi.org/10.17660/actahortic.2024.1413.7
  14. Sarshayeva M., Tashkenbayeva A., Bilibayeva A., Irsaliyeva Z., Ustemirova A. (2024). Technological aspects of in vitro propagation of organic strawberries // SABRAO Journal of Breeding & Genetics. Т. 56. №. 1. Pp. 246. http://doi.org/10.54910/sabrao2024.56.1.22
  15. Naing, A.H., Kim, S.H., Chung, M.Y., Park, S.K., Kim, C.K. (2019). In vitro propagation method for production of morphologically and genetically stable plants of different strawberry cultivars //Plant methods. Т. 15. №. 1. Pp. 36. https://doi.org/10.1186/s13007-019-0421-0
  16. Kabylbekova B.Z., Chukanova N.A., Turdiyev T.T., Rymkhanova N., Kovalchuk I.Y. (2019). Optimizatsiya kloniroavniya in vitro razlichnykh genotipov yabloni //Experimental Biology. Т. 80. №. 3. Pp. 48– 57. https://doi.org/10.26577/eb-2019-3-b5 [in Rus]
  17. Kryukov L.A., Vodolazhsky D.I., Kamenetsky-Goldstein R. (2022). Micropropagation of grapevine and strawberry from south russia: rapid production and genetic uniformity // Agronomy. Т. 12. №. 2. Pp. 308. https://doi.org/10.3390/agronomy12020308

Citation Links

[1]2026. OPTIMIZATION OF THE CLONAL MICROPROPAGATION METHOD FOR OBTAINING VI RUS-FREE PLANTING MATERIAL OF GARDEN STRAWBERRIES (FRAGARIA×ANANASSA). Izdenister natigeler. 28, 1(109) (Feb. 2026), 93–105. DOI:https://doi.org/10.37884/1-2026/10.
(1)OPTIMIZATION OF THE CLONAL MICROPROPAGATION METHOD FOR OBTAINING VI RUS-FREE PLANTING MATERIAL OF GARDEN STRAWBERRIES (FRAGARIA×ANANASSA). Izdenister natigeler 2026, 28 (1(109), 93-105. https://doi.org/10.37884/1-2026/10.
OPTIMIZATION OF THE CLONAL MICROPROPAGATION METHOD FOR OBTAINING VI RUS-FREE PLANTING MATERIAL OF GARDEN STRAWBERRIES (FRAGARIA×ANANASSA). (2026). Izdenister Natigeler, 28(1(109), 93-105. https://doi.org/10.37884/1-2026/10
OPTIMIZATION OF THE CLONAL MICROPROPAGATION METHOD FOR OBTAINING VI RUS-FREE PLANTING MATERIAL OF GARDEN STRAWBERRIES (FRAGARIA×ANANASSA). Izdenister natigeler, [S. l.], v. 28, n. 1(109), p. 93–105, 2026. DOI: 10.37884/1-2026/10. Disponível em: https://natsus.kaznaru.edu.kz/index.php/research/article/view/1109. Acesso em: 15 sep. 2026.
“OPTIMIZATION OF THE CLONAL MICROPROPAGATION METHOD FOR OBTAINING VI RUS-FREE PLANTING MATERIAL OF GARDEN STRAWBERRIES (FRAGARIA×ANANASSA)”. 2026. Izdenister Natigeler 28 (1(109): 93-105. https://doi.org/10.37884/1-2026/10.
“OPTIMIZATION OF THE CLONAL MICROPROPAGATION METHOD FOR OBTAINING VI RUS-FREE PLANTING MATERIAL OF GARDEN STRAWBERRIES (FRAGARIA×ANANASSA)” (2026) Izdenister natigeler, 28(1(109), pp. 93–105. doi:10.37884/1-2026/10.
[1]“OPTIMIZATION OF THE CLONAL MICROPROPAGATION METHOD FOR OBTAINING VI RUS-FREE PLANTING MATERIAL OF GARDEN STRAWBERRIES (FRAGARIA×ANANASSA)”, Izdenister natigeler, vol. 28, no. 1(109), pp. 93–105, Feb. 2026, doi: 10.37884/1-2026/10.
“OPTIMIZATION OF THE CLONAL MICROPROPAGATION METHOD FOR OBTAINING VI RUS-FREE PLANTING MATERIAL OF GARDEN STRAWBERRIES (FRAGARIA×ANANASSA)”. Izdenister Natigeler, vol. 28, no. 1(109), Feb. 2026, pp. 93-105, https://doi.org/10.37884/1-2026/10.
“OPTIMIZATION OF THE CLONAL MICROPROPAGATION METHOD FOR OBTAINING VI RUS-FREE PLANTING MATERIAL OF GARDEN STRAWBERRIES (FRAGARIA×ANANASSA)”. Izdenister natigeler 28, no. 1(109) (February 27, 2026): 93–105. Accessed September 15, 2026. https://natsus.kaznaru.edu.kz/index.php/research/article/view/1109.
1.OPTIMIZATION OF THE CLONAL MICROPROPAGATION METHOD FOR OBTAINING VI RUS-FREE PLANTING MATERIAL OF GARDEN STRAWBERRIES (FRAGARIA×ANANASSA). Izdenister natigeler [Internet]. 2026 Feb. 27 [cited 2026 Sep. 15];28(1(109):93-105. Available from: https://natsus.kaznaru.edu.kz/index.php/research/article/view/1109
1.OPTIMIZATION OF THE CLONAL MICROPROPAGATION METHOD FOR OBTAINING VI RUS-FREE PLANTING MATERIAL OF GARDEN STRAWBERRIES (FRAGARIA×ANANASSA). Izdenister natigeler. 2026;28(1(109):93-105. doi:10.37884/1-2026/10