ИДЕНТИФИКАЦИЯ ВИРУСНЫХ ЗАБОЛЕВАНИИ ЯБЛОНИ В КАЗАХСТАНСКИХ САДАХ МЕТОДОМ ОТ-ПЦР-РВ

Айгуль Маденова; Динара Калдыбаева; Райгүл Әбдікәрімова; Аңсаған Қуан

doi:10.37884/3-2024/11

Abstract

In Kazakhstan, apples dominate the country’s agro-economic niche, as evidenced by the huge gardens of crops. However, the widespread usage of rootstocks derived from virus-infected maternal and vegetative reproduction causes apples to have high levels of viral infection, which is widely used as a grafting among varieties. To fight viruses and viroids in plants, a mass, rapid, and accurate diagnostic examination is the most effective method. Therefore, this study aimed to investigate the spread of two viruses ACLSV, ASPV and viroid AHVd in three regions of Kazakhstan. Virus detection is based on a combination of reverse transcription methods and a multiplexed polymerase chain reaction with real-time results (RT-PCR). Among 49 samples, RT-PCR in real time showed positive results of 18.4% ACLSV, 10.2% AHVd, and 51.02% ASPV. The results can be used for healing and to obtain virus-free stock material. Thus, the problem of providing the market of Kazakhstan with quality domestic planting material for trees has been solved.

apple, rootstocks, variety, viral diseases, viroid, ACLSV, ASPV, AHVd, RNA, RT-PCR.

01 Introduction

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02 References

1 Galymbek K., A. K. Madenova, S. B. Bakirov, B. Zh Kabylbekova, A. Irkitbay, Zh Aitymbet, D. I. Kaldybayeva, R. Abdikarimova, and Bolat Munira. Monitoring the distribution and development of apple scab (Venturia inaequalis) and powdery mildew (Podosphaera leucotricha) disease in the southern and southeast regions of Kazakhstan //Bulletin of the Karaganda university Biology. Medicine. Geography series. – 2023. – Т. 110. – №. 2. – С. 38-45.
2 Shi W., Yao R., Sunwu R., Huang K., Liu Z., Li X., Yang Y., Wang J. Incidence and molecular identification of apple necrotic mosaic virus (ApNMV) in Southwest China //Plants. – 2020. – Т. 9. – №. 4. – С. 415.
3 EFSA Panel on Plant Health (EFSA PLH Panel), Bragard C., Dehnen-Schmutz K., Gonthier P., Jacques M., Jaques Miret J.A., Justesen A.F., MacLeod A., Magnusson C.S., Milonas P., et al. Pest categorisation of the Ralstonia solanacearum species complex //EFSA Journal. – 2019. – Т. 17. – №. 2. – С. e05618.
4 Wright A. A., Cross A. R., Harper S. J. A bushel of viruses: Identification of seventeen novel putative viruses by RNA-seq in six apple trees //PLoS One. – 2020. – Т. 15. – №. 1. – С. e0227669.
5 Saade M., Aparicio, F., Sanchez-Navarro, J. A., Herranz, M. C., Myrta, A., Di Terlizzi, B., Pallas, V. Simultaneous detection of the three ilarviruses affecting stone fruit trees by nonisotopic molecular hybridization and multiplex reverse-transcription polymerase chain reaction //Phytopathology. – 2000. – Т. 90. – №. 12. – С. 1330-1336.
6 Várallyay E., Přibylová J., Galbacs Z.N., Jahan A., Varga T., Špak J., Lenz O., Fránová J., Sedlák J., Koloniuk I. Detection of Apple hammerhead viroid, Apple Luteovirus 1 and Citrus Concave gum-associated virus in apple propagation materials and orchards in the Czech Republic and Hungary //Viruses. – 2022. – Т. 14. – №. 11. – С. 2347.
7 Hassan M., Polak J., Paprštein F. Detection and distribution of four pome fruit viruses in germplasm collection in the Czech Republic //XX International Symposium on Virus and Virus-Like Diseases of Temperate Fruit Crops-Fruit Tree Diseases 781. – 2006. – С. 113-118.
8 Yoshikawa N., Yaegashi H. Betaflexiviruses (Betaflexiviridae) //Encyclopedia of Virology. – 2021. – С. 229-238.
9 Zhang Z., Qi S., Tang N., Zhang X., Chen S., Zhu P., Wu Q. Discovery of replicating circular RNAs by RNA-seq and computational algorithms //PLoS pathogens. – 2014. – Т. 10. – №. 12. – С. E1004553.
10 Serra P., Messmer A., Sanderson D., James D., Flores R. Apple hammerhead viroid-like RNA is a bona fide viroid: Autonomous replication and structural features support its inclusion as a new member in the genus Pelamoviroid //Virus research. – 2018. – Т. 249. – С. 8-15.
11 Messmer A., Sanderson D., Braun G., Serra P., Flores R., James D. Molecular and phylogenetic identification of unique isolates of hammerhead viroid-like RNA from ‘Pacific Gala’apple (Malus domestica) in Canada //Canadian Journal of Plant Pathology. – 2017. – Т. 39. – №. 3. – С. 342-353.
12 Szostek S. A., Wright A. A., Harper S. J. First report of apple hammerhead viroid in the United States, Japan, Italy, Spain, and New Zealand //Plant Disease. – 2018. – Т. 102. – №. 12. – С. 2670-2670.
13 Hamdi I., Soltani R., Baraket G., Varsani A., Najar A. First report of apple hammerhead viroid infecting ‘Richared Delicious’ apple (Malus domestica) in Tunisia //Journal of Plant Pathology. – 2022. – Т. 104. – №. 2. – С. 811-812.
14 Chiumenti M., Navarro B., Venerito P., Civita F., Minafra A., Di Serio F. Molecular variability of apple hammerhead viroid from Italian apple varieties supports the relevance in vivo of its branched conformation stabilized by a kissing loop interaction //Virus research. – 2019. – Т. 270. – С. 197644.
15 Pareta N. F., Lateur M., Steyer S., Blouin A. G., Massart S. First reports of Apple luteovirus 1, Apple rubodvirus 1 and Apple hammerhead viroid infecting apples in Belgium //New Disease Reports. – 2022. – Т. 45. – №2.
16 Heo S., Chung Y. S. Rapid real-time detection method of ACLSV and ASSVd for apple quarantine field //Plant Biotechnology Reports. – 2021. – Т. 15. – С. 187-195.
17 Gritsenko D. A., Aubakirova K. P., Voitsekhovskiy I., Soldatova I., Galiakparov N. N. Simultaneous detection of five apple viruses by RT-PCR //International Journal of Biology and Chemistry. – 2020. – Т. 13. – №. 1. – С. 129-134.
18 Madenova A., Aitymbet Z., Bolat M., Kaldybayeva D., Galymbek K., Kuan A., Kabylbekova B., Irkitbay A., Yeszhanov T., Bakirov S., Sapakhova Z. Screening of Apple Cultivars for Scab Resistance in Kazakhstan //Horticulturae. – 2024. – Т. 10. – №. 2. – С. 184.

Citation Links

[1]2023. IDENTIFICATION OF APPLE VIRAL DISEASES IN KAZAKHSTAN ORCHARDS BY RT-PCR METHOD. Izdenister natigeler. 3(103) (Sep. 2023), 97–108. DOI:https://doi.org/10.37884/3-2024/11.

(1)IDENTIFICATION OF APPLE VIRAL DISEASES IN KAZAKHSTAN ORCHARDS BY RT-PCR METHOD. Izdenister natigeler 2023, No. 3(103), 97-108. https://doi.org/10.37884/3-2024/11.

IDENTIFICATION OF APPLE VIRAL DISEASES IN KAZAKHSTAN ORCHARDS BY RT-PCR METHOD. (2023). Izdenister Natigeler, 3(103), 97-108. https://doi.org/10.37884/3-2024/11

IDENTIFICATION OF APPLE VIRAL DISEASES IN KAZAKHSTAN ORCHARDS BY RT-PCR METHOD. Izdenister natigeler, [S. l.], n. 3(103), p. 97–108, 2023. DOI: 10.37884/3-2024/11. Disponível em: https://natsus.kaznaru.edu.kz/index.php/research/article/view/695. Acesso em: 15 sep. 2026.

“IDENTIFICATION OF APPLE VIRAL DISEASES IN KAZAKHSTAN ORCHARDS BY RT-PCR METHOD”. 2023. Izdenister Natigeler, no. 3(103) (September): 97-108. https://doi.org/10.37884/3-2024/11.

“IDENTIFICATION OF APPLE VIRAL DISEASES IN KAZAKHSTAN ORCHARDS BY RT-PCR METHOD” (2023) Izdenister natigeler, (3(103), pp. 97–108. doi:10.37884/3-2024/11.

[1]“IDENTIFICATION OF APPLE VIRAL DISEASES IN KAZAKHSTAN ORCHARDS BY RT-PCR METHOD”, Izdenister natigeler, no. 3(103), pp. 97–108, Sep. 2023, doi: 10.37884/3-2024/11.

“IDENTIFICATION OF APPLE VIRAL DISEASES IN KAZAKHSTAN ORCHARDS BY RT-PCR METHOD”. Izdenister Natigeler, no. 3(103), Sept. 2023, pp. 97-108, https://doi.org/10.37884/3-2024/11.

“IDENTIFICATION OF APPLE VIRAL DISEASES IN KAZAKHSTAN ORCHARDS BY RT-PCR METHOD”. Izdenister natigeler, no. 3(103) (September 30, 2023): 97–108. Accessed September 15, 2026. https://natsus.kaznaru.edu.kz/index.php/research/article/view/695.

1.IDENTIFICATION OF APPLE VIRAL DISEASES IN KAZAKHSTAN ORCHARDS BY RT-PCR METHOD. Izdenister natigeler [Internet]. 2023 Sep. 30 [cited 2026 Sep. 15];(3(103):97-108. Available from: https://natsus.kaznaru.edu.kz/index.php/research/article/view/695

1.IDENTIFICATION OF APPLE VIRAL DISEASES IN KAZAKHSTAN ORCHARDS BY RT-PCR METHOD. Izdenister natigeler. 2023;(3(103):97-108. doi:10.37884/3-2024/11

[1] 1 Galymbek K., A. K. Madenova, S. B. Bakirov, B. Zh Kabylbekova, A. Irkitbay, Zh Aitymbet, D. I. Kaldybayeva, R. Abdikarimova, and Bolat Munira. Monitoring the distribution and development of apple scab (Venturia inaequalis) and powdery mildew (Podosphaera leucotricha) disease in the southern and southeast regions of Kazakhstan //Bulletin of the Karaganda university Biology. Medicine. Geography series. – 2023. – Т. 110. – №. 2. – С. 38-45.

[2] 2 Shi W., Yao R., Sunwu R., Huang K., Liu Z., Li X., Yang Y., Wang J. Incidence and molecular identification of apple necrotic mosaic virus (ApNMV) in Southwest China //Plants. – 2020. – Т. 9. – №. 4. – С. 415.

[3] 3 EFSA Panel on Plant Health (EFSA PLH Panel), Bragard C., Dehnen-Schmutz K., Gonthier P., Jacques M., Jaques Miret J.A., Justesen A.F., MacLeod A., Magnusson C.S., Milonas P., et al. Pest categorisation of the Ralstonia solanacearum species complex //EFSA Journal. – 2019. – Т. 17. – №. 2. – С. e05618.

[4] 4 Wright A. A., Cross A. R., Harper S. J. A bushel of viruses: Identification of seventeen novel putative viruses by RNA-seq in six apple trees //PLoS One. – 2020. – Т. 15. – №. 1. – С. e0227669.

[5] 5 Saade M., Aparicio, F., Sanchez-Navarro, J. A., Herranz, M. C., Myrta, A., Di Terlizzi, B., Pallas, V. Simultaneous detection of the three ilarviruses affecting stone fruit trees by nonisotopic molecular hybridization and multiplex reverse-transcription polymerase chain reaction //Phytopathology. – 2000. – Т. 90. – №. 12. – С. 1330-1336.

[6] 6 Várallyay E., Přibylová J., Galbacs Z.N., Jahan A., Varga T., Špak J., Lenz O., Fránová J., Sedlák J., Koloniuk I. Detection of Apple hammerhead viroid, Apple Luteovirus 1 and Citrus Concave gum-associated virus in apple propagation materials and orchards in the Czech Republic and Hungary //Viruses. – 2022. – Т. 14. – №. 11. – С. 2347.

[7] 7 Hassan M., Polak J., Paprštein F. Detection and distribution of four pome fruit viruses in germplasm collection in the Czech Republic //XX International Symposium on Virus and Virus-Like Diseases of Temperate Fruit Crops-Fruit Tree Diseases 781. – 2006. – С. 113-118.

[8] 8 Yoshikawa N., Yaegashi H. Betaflexiviruses (Betaflexiviridae) //Encyclopedia of Virology. – 2021. – С. 229-238.

[9] 9 Zhang Z., Qi S., Tang N., Zhang X., Chen S., Zhu P., Wu Q. Discovery of replicating circular RNAs by RNA-seq and computational algorithms //PLoS pathogens. – 2014. – Т. 10. – №. 12. – С. E1004553.

[10] 10 Serra P., Messmer A., Sanderson D., James D., Flores R. Apple hammerhead viroid-like RNA is a bona fide viroid: Autonomous replication and structural features support its inclusion as a new member in the genus Pelamoviroid //Virus research. – 2018. – Т. 249. – С. 8-15.

[11] 11 Messmer A., Sanderson D., Braun G., Serra P., Flores R., James D. Molecular and phylogenetic identification of unique isolates of hammerhead viroid-like RNA from ‘Pacific Gala’apple (Malus domestica) in Canada //Canadian Journal of Plant Pathology. – 2017. – Т. 39. – №. 3. – С. 342-353.

[12] 12 Szostek S. A., Wright A. A., Harper S. J. First report of apple hammerhead viroid in the United States, Japan, Italy, Spain, and New Zealand //Plant Disease. – 2018. – Т. 102. – №. 12. – С. 2670-2670.

[13] 13 Hamdi I., Soltani R., Baraket G., Varsani A., Najar A. First report of apple hammerhead viroid infecting ‘Richared Delicious’ apple (Malus domestica) in Tunisia //Journal of Plant Pathology. – 2022. – Т. 104. – №. 2. – С. 811-812.

[14] 14 Chiumenti M., Navarro B., Venerito P., Civita F., Minafra A., Di Serio F. Molecular variability of apple hammerhead viroid from Italian apple varieties supports the relevance in vivo of its branched conformation stabilized by a kissing loop interaction //Virus research. – 2019. – Т. 270. – С. 197644.

[15] 15 Pareta N. F., Lateur M., Steyer S., Blouin A. G., Massart S. First reports of Apple luteovirus 1, Apple rubodvirus 1 and Apple hammerhead viroid infecting apples in Belgium //New Disease Reports. – 2022. – Т. 45. – №2.

[16] 16 Heo S., Chung Y. S. Rapid real-time detection method of ACLSV and ASSVd for apple quarantine field //Plant Biotechnology Reports. – 2021. – Т. 15. – С. 187-195.

[17] 17 Gritsenko D. A., Aubakirova K. P., Voitsekhovskiy I., Soldatova I., Galiakparov N. N. Simultaneous detection of five apple viruses by RT-PCR //International Journal of Biology and Chemistry. – 2020. – Т. 13. – №. 1. – С. 129-134.

[18] 18 Madenova A., Aitymbet Z., Bolat M., Kaldybayeva D., Galymbek K., Kuan A., Kabylbekova B., Irkitbay A., Yeszhanov T., Bakirov S., Sapakhova Z. Screening of Apple Cultivars for Scab Resistance in Kazakhstan //Horticulturae. – 2024. – Т. 10. – №. 2. – С. 184.