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AGRICULTURE, AGROCHEMICAL, FEED PRODUCTION, AGROECOLOGY

OPTIMIZATION OF GROWTH REGULATORS IN LIQUID NUTRIENT MEDIUM FOR MICROTUBERS PRODUCTION IN BIOREACTOR

Dias Daurov 1 , Zhanar Аbilda 1 , Ruslan Kalendar 2 , Dmitry Volkov 1 , Rakhim Kanat 1 , Asel Argynbaeva 1 , Malika Shamekova 1

1 Institute of Plant Biology and Biotechnology; 2 Private Institution “National Laboratory Astana”

doi.org/10.37884/3-2024/26 pp. 230-239 Admitted 16.07.2024 Published 30.09.2024

Abstract

The aim of the study was to optimize liquid nutrient media for the growth and development of potato plants of Colombo variety and the production of microtubers from them in a temporary immersion bioreactor.
            In the study, optimization of MS nutrient media by phytohormones for growth and development of potato plant explants and subsequent formation of microtubers from them was carried out.
            The results showed that the most optimal liquid MS medium for obtaining well-developed plants was the nutrient medium containing hormones GA (0.1 mg/L) and IBA (0.5 mg/L), and the medium containing hormones IBA (2 mg/L) and Kinetin (2 mg/L) for microtubers formation.

potato, phytohormones, kinetin, IAA, IBA, bioreactor, microtubers.

01 Introduction

The full text of the article is available for download in PDF format on the right panel.

02 References

  1. 1.Мухаметов, А., Даутканова, Д., Даутқанов , Н. ., Даулетбекова, А., & Шаймерденова, Ж. (2023). ПРОИЗВОДСТВО СЕМЕННОГО КАРТОФЕЛЯ В КАЗАХСТАНЕ. Izdenister Natigeler, (2 (98), 412–422. https://doi.org/10.37884/2-2023/41
  2. 2.Hulscher, M., Krijgsheld, H., & Jongedijk, E. (1996). MASS PROPAGATION OF POTATO MICROTUBERS IN JAR FERMENTORS. Acta Horticulturae, 440, 533–538. https://doi.org/10.17660/actahortic.1996.440.93
  3. 3.Engels, J. M. M., & Ebert, A. W. (2021). A Critical Review of the Current Global Ex Situ Conservation System for Plant Agrobiodiversity. I. History of the Development of the Global System in the Context of the Political/Legal Framework and Its Major Conservation Components. Plants (Basel, Switzerland), 10(8), 1557. https://doi.org/10.3390/plants10081557
  4. 4.Kikowska, M., Danek, K., Gornowicz-Porowska, J., & Thiem, B. (2022). Application of temporary immersion system RITA® for efficient biomass multiplication and production of artificial seeds for ex situ conservation of Linnaea borealis L. Plant Cell, Tissue and Organ Culture, 151(3), 673–680. https://doi.org/10.1007/s11240-022-02381-7
  5. 5.Plant Hormones - Recent Advances, New Perspectives and Applications. (2022). In IntechOpen eBooks. https://doi.org/10.5772/intechopen.95608
  6. 6.Santner, A., Calderon-Villalobos, L. I. A., & Estelle, M. (2009). Plant hormones are versatile chemical regulators of plant growth. Nature Chemical Biology, 5(5), 301–307. https://doi.org/10.1038/nchembio.165
  7. 7.Daurov, D., Daurova, A., Karimov, A., Tolegenova, D., Volkov, D., Raimbek, D., Zhambakin, K., & Shamekova, M. (2020). Determining Effective Methods of Obtaining Virus-Free Potato for Cultivation in Kazakhstan. American Journal of Potato Research, 97(4), 367–375. https://doi.org/10.1007/s12230-020-09787-z
  8. 8.Murashige, T., & Skoog, F. (1962). A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum, 15(3), 473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  9. 9.Saidi, A., & Hajibarat, Z. (2021). Phytohormones: plant switchers in developmental and growth stages in potato. Journal of Genetic Engineering and Biotechnology /Journal of Genetic Engineering and Biotechnology, 19(1), 89. https://doi.org/10.1186/s43141-021-00192-5
  10. 10.Brenner, W.G., Romanov, G.A., Köllmer, I., Bürkle, L. and Schmülling, T. (2005), Immediate-early and delayed cytokinin response genes of Arabidopsis thaliana identified by genome-wide expression profiling reveal novel cytokinin-sensitive processes and suggest cytokinin action through transcriptional cascades. The Plant Journal, 44: 314-333. https://doi.org/10.1111/j.1365-313X.2005.02530.x
  11. 11.Gizatullina A.T., Stasevski Z., Gimaeva E.A., Safiullina G.F. (2019) Potato (Solanum tuberosum L.) cv. Nevskij microtuber formation features in in vitro culture. Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki, vol. 161, no. 3, pp. 375–384. doi: 10.26907/2542-064X.2019.3.375-384. (In Russian)

Citation Links

[1]2024. OPTIMIZATION OF GROWTH REGULATORS IN LIQUID NUTRIENT MEDIUM FOR MICROTUBERS PRODUCTION IN BIOREACTOR. Izdenister natigeler. 3(103) (Sep. 2024), 230–239. DOI:https://doi.org/10.37884/3-2024/26.
(1)OPTIMIZATION OF GROWTH REGULATORS IN LIQUID NUTRIENT MEDIUM FOR MICROTUBERS PRODUCTION IN BIOREACTOR. Izdenister natigeler 2024, No. 3(103), 230-239. https://doi.org/10.37884/3-2024/26.
OPTIMIZATION OF GROWTH REGULATORS IN LIQUID NUTRIENT MEDIUM FOR MICROTUBERS PRODUCTION IN BIOREACTOR. (2024). Izdenister Natigeler, 3(103), 230-239. https://doi.org/10.37884/3-2024/26
OPTIMIZATION OF GROWTH REGULATORS IN LIQUID NUTRIENT MEDIUM FOR MICROTUBERS PRODUCTION IN BIOREACTOR. Izdenister natigeler, [S. l.], n. 3(103), p. 230–239, 2024. DOI: 10.37884/3-2024/26. Disponível em: https://natsus.kaznaru.edu.kz/index.php/research/article/view/654. Acesso em: 15 sep. 2026.
“OPTIMIZATION OF GROWTH REGULATORS IN LIQUID NUTRIENT MEDIUM FOR MICROTUBERS PRODUCTION IN BIOREACTOR”. 2024. Izdenister Natigeler, no. 3(103) (September): 230-39. https://doi.org/10.37884/3-2024/26.
“OPTIMIZATION OF GROWTH REGULATORS IN LIQUID NUTRIENT MEDIUM FOR MICROTUBERS PRODUCTION IN BIOREACTOR” (2024) Izdenister natigeler, (3(103), pp. 230–239. doi:10.37884/3-2024/26.
[1]“OPTIMIZATION OF GROWTH REGULATORS IN LIQUID NUTRIENT MEDIUM FOR MICROTUBERS PRODUCTION IN BIOREACTOR”, Izdenister natigeler, no. 3(103), pp. 230–239, Sep. 2024, doi: 10.37884/3-2024/26.
“OPTIMIZATION OF GROWTH REGULATORS IN LIQUID NUTRIENT MEDIUM FOR MICROTUBERS PRODUCTION IN BIOREACTOR”. Izdenister Natigeler, no. 3(103), Sept. 2024, pp. 230-9, https://doi.org/10.37884/3-2024/26.
“OPTIMIZATION OF GROWTH REGULATORS IN LIQUID NUTRIENT MEDIUM FOR MICROTUBERS PRODUCTION IN BIOREACTOR”. Izdenister natigeler, no. 3(103) (September 30, 2024): 230–239. Accessed September 15, 2026. https://natsus.kaznaru.edu.kz/index.php/research/article/view/654.
1.OPTIMIZATION OF GROWTH REGULATORS IN LIQUID NUTRIENT MEDIUM FOR MICROTUBERS PRODUCTION IN BIOREACTOR. Izdenister natigeler [Internet]. 2024 Sep. 30 [cited 2026 Sep. 15];(3(103):230-9. Available from: https://natsus.kaznaru.edu.kz/index.php/research/article/view/654
1.OPTIMIZATION OF GROWTH REGULATORS IN LIQUID NUTRIENT MEDIUM FOR MICROTUBERS PRODUCTION IN BIOREACTOR. Izdenister natigeler. 2024;(3(103):230-239. doi:10.37884/3-2024/26