Menu
AGRICULTURE, AGROCHEMICAL, FEED PRODUCTION, AGROECOLOGY

PRODUCTION OF HEALTHY PLANTING MATERIAL OF GARDEN STRAWBERRIES OF THE BEST VARIETIES OF THE WORLD COLLECTION FOR THE CREATION OF ORIGINAL BASIC NURSERY PLANTS

Akerke Tashkenbayeva 1 , Moldir Sarshaeva 1 , Zhansaya Matay 1

1 Kazakh Scientific Research Institute of Fruit and Vegetable Growing LLP

doi.org/10.37884/4-2023/18 pp. 159-166 Admitted 04.10.2023 Published 06.12.2023

Abstract

The article presents experimental data on the production of improved planting material of four varieties of garden strawberries in the culture of apical meristems for the creation of basic and reproductive uterine plantings, accelerated introduction into production of the best varieties from the world collection. selected strawberry varieties were introduced into the tissue culture with the improvement of the technological scheme of clonal micro-propagation specifically for each variety at the stages of explant sterilization, introduction into tissue culture, primary regeneration of apexes, proliferation and rhizogenesis with genotype preservation. A sufficient amount of material has been propagated in tissue culture and the adaptation period has been improved – during the transition of plants in vitroex vitro. Microcloned plants in the greenhouse were grown to standard sizes of the basic category and replicated to basic sockets. The biotechnology of microclonal reproduction of strawberries in vitro and obtaining planting material free from viral and mycoplasma infections has been developed. The elements of the technology of clonal micropropagation of promising varieties of strawberry in vitro have been optimized. The reproduction coefficient for 5 passages averaged from 50 for the Sabrina variety to 150 for the Black Prince variety.  The rate of formation of the root system depended on varietal characteristics. The Black Prince variety was distinguished by a high rate of root formation - 25-30 days after passage to the root medium, then the Sabrina and Malvina varieties -35-40 days and the San Andres variety - 45-55 days.

strawberry garden, clonal micro-reproduction in vitro, ex vitro adaptation, basic queen cell, variety, tissue culture, nutrient medium, berry crops

01 Introduction

The full text of the article is available for download in PDF format on the right panel.

02 References

  1. 1 Долгих С.Г. Технология производства безвирусного посадочного материала плодовых, ягодных культур и винограда (Рекомендации). – 2020.- 57 с.
  2. 2 Pang W. Q. et al. Establishment of an efficient micropropagation protocol for Cameron Highlands White Strawberry (Fragaria x ananassa) using light emitting diodes (LEDs) system //South African Journal of Botany. – 2023. – Т. 157. – С. 189-200.
  3. 3 Сковородников Д.Н., Леонова Н.В., Андронова Н.В. Влияние состава питательной среды на эффективность размножения земляники in vitro // Вестник Орловского государственного аграрного университета, 2013. – Т. 40. – № 1. – С. 89–92.
  4. 4 Rojas, P.; Almada, R.D.; Sandoval, C.; Keller, K.E.; Martin, R.R.; Caligari, P.D.S. Occurrence of aphidborne viruses in southernmost South American populations of Fragaria chiloensis ssp. chiloensis. Plant Pathol. 2013, 62, 428–435.
  5. 5 Quiroz, K.A.; Berríos, M.; Carrasco, B.; Retamales, J.B.; Caligari, P.D.S.; García-Gonzáles, R. Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.). Biol. Res. 2017, 50, 20.
  6. 6 Moradi, K.; Otroshy, M.; Azimi, M.R. Micropropagation of strawberry by multiple shoots regeneration tissue cultures. Int. J. Agric. Technol. 2011, 7, 1755–1763.
  7. 7 Мацнева О. В., Ташматова Л. В. Клональное микроразмножение земляники–перспективный метод современного питомниководства (обзор) //Современное садоводство–Contemporary horticulture. – 2019. – №. 4. – С. 113-119.
  8. 8 Разумников Н.А. Содержание элеутерозидов и микроэлементов в корневищах и корнях элеутерококка колючего//Нива Поволжья/Промышленные биотехнологии. -2011.- С.90-94
  9. 9 Сулейманова С.Д. Микроклональное размножение плодовых культур // Wschodnioeuropean Czasopismo Naukove. 2016. №11, Ч.2. С.47-54.
  10. 10 Жеміс-жидек дақылдары мен жүзімнің вируссыз отырғызу материалын өндіру және органикалық баққа өндіру технологиясы (Ұсынымдар) / А.К Ташкенбаева, Ж.С Ирсалиева, С.Г Долгих – Алматы: 2023.-60 бет

Citation Links

[1]2023. PRODUCTION OF HEALTHY PLANTING MATERIAL OF GARDEN STRAWBERRIES OF THE BEST VARIETIES OF THE WORLD COLLECTION FOR THE CREATION OF ORIGINAL BASIC NURSERY PLANTS. Izdenister natigeler. 4 (100) (Dec. 2023), 159–166. DOI:https://doi.org/10.37884/4-2023/18.
(1)PRODUCTION OF HEALTHY PLANTING MATERIAL OF GARDEN STRAWBERRIES OF THE BEST VARIETIES OF THE WORLD COLLECTION FOR THE CREATION OF ORIGINAL BASIC NURSERY PLANTS. Izdenister natigeler 2023, No. 4 (100), 159-166. https://doi.org/10.37884/4-2023/18.
PRODUCTION OF HEALTHY PLANTING MATERIAL OF GARDEN STRAWBERRIES OF THE BEST VARIETIES OF THE WORLD COLLECTION FOR THE CREATION OF ORIGINAL BASIC NURSERY PLANTS. (2023). Izdenister Natigeler, 4 (100), 159-166. https://doi.org/10.37884/4-2023/18
PRODUCTION OF HEALTHY PLANTING MATERIAL OF GARDEN STRAWBERRIES OF THE BEST VARIETIES OF THE WORLD COLLECTION FOR THE CREATION OF ORIGINAL BASIC NURSERY PLANTS. Izdenister natigeler, [S. l.], n. 4 (100), p. 159–166, 2023. DOI: 10.37884/4-2023/18. Disponível em: https://natsus.kaznaru.edu.kz/index.php/research/article/view/391. Acesso em: 15 sep. 2026.
“PRODUCTION OF HEALTHY PLANTING MATERIAL OF GARDEN STRAWBERRIES OF THE BEST VARIETIES OF THE WORLD COLLECTION FOR THE CREATION OF ORIGINAL BASIC NURSERY PLANTS”. 2023. Izdenister Natigeler, no. 4 (100) (December): 159-66. https://doi.org/10.37884/4-2023/18.
“PRODUCTION OF HEALTHY PLANTING MATERIAL OF GARDEN STRAWBERRIES OF THE BEST VARIETIES OF THE WORLD COLLECTION FOR THE CREATION OF ORIGINAL BASIC NURSERY PLANTS” (2023) Izdenister natigeler, (4 (100), pp. 159–166. doi:10.37884/4-2023/18.
[1]“PRODUCTION OF HEALTHY PLANTING MATERIAL OF GARDEN STRAWBERRIES OF THE BEST VARIETIES OF THE WORLD COLLECTION FOR THE CREATION OF ORIGINAL BASIC NURSERY PLANTS”, Izdenister natigeler, no. 4 (100), pp. 159–166, Dec. 2023, doi: 10.37884/4-2023/18.
“PRODUCTION OF HEALTHY PLANTING MATERIAL OF GARDEN STRAWBERRIES OF THE BEST VARIETIES OF THE WORLD COLLECTION FOR THE CREATION OF ORIGINAL BASIC NURSERY PLANTS”. Izdenister Natigeler, no. 4 (100), Dec. 2023, pp. 159-66, https://doi.org/10.37884/4-2023/18.
“PRODUCTION OF HEALTHY PLANTING MATERIAL OF GARDEN STRAWBERRIES OF THE BEST VARIETIES OF THE WORLD COLLECTION FOR THE CREATION OF ORIGINAL BASIC NURSERY PLANTS”. Izdenister natigeler, no. 4 (100) (December 6, 2023): 159–166. Accessed September 15, 2026. https://natsus.kaznaru.edu.kz/index.php/research/article/view/391.
1.PRODUCTION OF HEALTHY PLANTING MATERIAL OF GARDEN STRAWBERRIES OF THE BEST VARIETIES OF THE WORLD COLLECTION FOR THE CREATION OF ORIGINAL BASIC NURSERY PLANTS. Izdenister natigeler [Internet]. 2023 Dec. 6 [cited 2026 Sep. 15];(4 (100):159-66. Available from: https://natsus.kaznaru.edu.kz/index.php/research/article/view/391
1.PRODUCTION OF HEALTHY PLANTING MATERIAL OF GARDEN STRAWBERRIES OF THE BEST VARIETIES OF THE WORLD COLLECTION FOR THE CREATION OF ORIGINAL BASIC NURSERY PLANTS. Izdenister natigeler. 2023;(4 (100):159-166. doi:10.37884/4-2023/18