OPTIMIZATION OF THE COMPOSITION OF THE NUTRIENT MEDIUM DURING REPRODUCTION OF ORGANIC STRAWBERRY IN VITRO

In world practice, clonal micro-propagation of strawberry is used for fast and effective reproduction of individual forms and varieties from a small amount of source material, in vitro selection at early stages of development. At the stages of introduction into the culture of strawberry plant tissues, active chlorine preparations were sterilized in the composition of sodium hypochlorite in various concentrations and exposures.

 For the development of regeneration and the acquisition of environmentally friendly strawberry seedlings, the amino acid proline at a concentration of 10 mg/ l, adenosine triphosphate (ATP) -10 mg / l, and vitamin C-ascorbic acid-10 mg / l were introduced into the nutrient medium in the exchange of growth regulators of chemical nature (cytokinins and auxins). It was found that the introduction of ascorbic acid at a concentration of 10 mg / l into the nutrient medium completely eliminates the phenolic oxidation of apexes at the stage of introduction into tissue culture and regeneration. The introduction of the amino acid proline and ATP into the nutrient medium increased regeneration by 10-15% for three varieties of strawberries.

After the proliferation stage, microcloned plants were transplanted to a nutrient medium for rooting. A large number of roots were formed on the Malvina variety. In the varieties Sabrina and Red Gontlit, these data were slightly lower than 5 and 7pieces. The height of the plants was distinguished by the Malvina variety 10pieces. The lowest plant height was characterized by the Sabrina and Red Gontlit varieties of 5 and 7pieces, respectively.

Rooted strawberry microplants were transplanted into ex vitro conditions for their adaptation.

A nutrient medium for obtaining organic strawberry seedlings for all stages of clonal micropropagation: introduction into tissue culture, regeneration, proliferation and rhizogenesis was developed.